Regulatory

Part:BBa_J100173

Designed by: Dustin Atchley   Group: Campbell M Lab   (2014-07-24)


Designed to optimize testing of fitness genes

This construct is meant to be inserted into a plasmid backbone (stringent plasmid recommended). It is a theophylline riboswitch (D) and contains BsaI sites. After digesting with BsaI a fitness gene with the appropriately designed sticky ends can be inserted and tested.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 240
    Illegal AgeI site found at 107
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 197
    Illegal BsaI.rc site found at 171


[edit]
Categories
Parameters
None